SRA

In many eukaryotes, mRNAs containing specific AU-rich motifs are regulated by proteins of the tristetraprolin (TTP) family, which bind to these motifs through a tandem zinc finger (TZF) domain. This binding leads to promotion of subsequent deadenylation and decay, partly through a conserved carboxyl-terminal CNOT1 binding domain. We explored the physiological role of the single TTP family member (TtpA) expressed in Dictyostelium discoideum. This study shows the effect of an insertional mutation within the TZF domain (ttpA-C240ins), which creates a null genotype, evaluated in amebae in growth medium during surface culture and liquid culture under axenic conditions. The mutant cells exhibited severe growth defects and a multinucleate phenotype during liquid culture, but not in surface culture. Overall design: Dictyostelium discoideum amebae (strain AX3) were grown in normal growth medium under axenic conditions in plastic flasks until they were approximately 80% confluent, or in liquid culture in the same medium until they reached approximately 2 million cells/ml. Six cultures of wild-type cells and six cultures of ttpA-C240ins cells were then frozen and used for RNA extraction and RNA-Seq analysis.